Peptide Reconstitution Guide

How to Reconstitute and Dose Peptide Vials

The amount of peptide in your vial determines how many doses you have— NOT the amount of water you add.

The 3-Step Process

Step 1 : Calculate the number of doses in your vial

Number of Doses = Vial Strength (mg) ÷ Desired Dose (mg)

Example: For this example, you will have a 10mg vial and require 1 mg doses.

      • 10mg vial ÷ 1mg dose = 10 doses per vial 
      • This never changes, regardless of the amount of water added.

Step 2 : Calculate Volume Per Dose

Volume per Dose (ml) = Total BAC Water Added (ml) ÷ Number of Doses

Examples:

      • Add 1ml water: 1ml ÷ 10 doses = 0.1ml per dose
      • Add 2ml water: 2ml ÷ 10 doses = 0.2ml per dose
      • Add 5ml water: 5ml ÷ 10 doses = 0.5ml per dose

Same 10 doses—just different volumes!

Step 3 : Convert to Insulin Syringe Units

1ml = 100 units on standard insulin syringe

Examples:

      • 0.1ml = 10 units
      • 0.2ml = 20 units
      • 0.3ml = 30 units

 

Complete Examples

 

Example 1: Standard 10mg Vial, 1mg Dose

Given : 10mg vial, want 10mg per dose, add 1ml of BAC water

Calculate:

      1. Doses: 10mg ÷ 1mg = 10 doses
      2. Volume: 1ml ÷ 10 doses = 0.1ml per dose
      3. Units: 0.1ml = 10 units on syringe

 

Example 2: Same Vial more water

Given : 10mg vial, want 10mg per dose, add 2ml of BAC water

Calculate:

      1. Doses: 10mg ÷ 1mg = 10 doses
      2. Volume: 2ml ÷ 10 doses = 0.2ml per dose
      3. Units: 0.2ml  = 20 units on syringe

 

How to Reconstitute Your Vial

 

What You Need:

  • Lyophilized peptide vial
  • Bacteriostatic water
  • Sterile syringe (3ml)
  • Alcohol prep pads

The Process:

1. Prepare - Clean rubber stoppers with alcohol swabs

2. Add Water

    • Draw BAC water into the syringe
    • Insert needle into peptide vial
    • Important: Aim needle at the vial wall, not directly at powder
    • Slowly inject water down the side of the vial

3. Mix

    • Never shake the vial!
    • Let dissolve naturally or gently swirl

       

Storage

Peptides are shipped as lyophilized powders and are stable at room temperature until reconstituted.

    • Refrigerate (2-8°C) after reconstitution
    • Use within 28 days
    • Label with date and concentration
    • Always use alcohol swabs before each use